Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate
All lanes ENO1 antibody at 1:10000
Secondary
Goat polyclonal to mouse IgG at 0.261ug/ml
Predicted band size: 47 KDa
Observed band size: 47 KDa
Exposure time: 10s

Western Blot
Positive WB detected in: HepG2 whole cell lysate at 20-¦g, 10-¦g, 5-¦g, 2.5-¦g, 1.25-¦g, 0.625-¦g
All lanes: ENO1 antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 KDa
Exposure time: 10s

Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: ENO1 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 KDa
Exposure time: 10s

Immunofluorescence staining of MCF-7 cells with CSB-MA007670A0m at 1:270, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4-¦C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).